V.One of the most important causes of the high mortality rate and low life expectancy of lung cancer is the detection at advanced stages. Thus, there is an urgent need for early diagnosis and the search of new selective biomarkers. Selenium is an important constituent of selenoproteins and a powerful antioxidant able to protect against cancer. In this work, the absolute quantification of selenium in selenoproteins and the total content in selenometabolites has been performed for the first time in serum from lung cancer patients (LC) and healthy controls (HC). To this end, a method for the simultaneous speciation of selenoproteins using size exclusion chromatography (SEC) and affinity chromatography (AF) with detection by ICP-QQQ-MS, and quantification by isotopic dilution (IDA) (SEC-AF-HPLC-SUID-ICP-QQQ-MS) was developed to determine the selenium concentration in eGPx, SEPP1 and SeAlb, as well as total selenometabolites, to find alterations that may serve as biomarkers of this disease. In the same way, a method based on anion-exchange chromatography coupled to ICP-QQQ-MS was developed to quantify selenometabolites (SeCys2, SeMeSeCys, SeMet, selenite and selenate) in the same LC and HC serum samples. The results showed that the averaged concentrations of selenium in eGPx, SeAlb and selenite were significantly higher in LC patients (LC (eGPx 21.24&nbsp;±&nbsp;0.77&nbsp;ng&nbsp;g-1; SeAlb 49.56&nbsp;±&nbsp;3.16&nbsp;ng&nbsp;g-1 and Se(IV) 6.20&nbsp;±&nbsp;1.22&nbsp;ng&nbsp;g-1) than in HC group (eGPx 16.96&nbsp;±&nbsp;0.53&nbsp;ng&nbsp;g-1; SeAlb 38.33&nbsp;±&nbsp;2.66&nbsp;ng&nbsp;g-1 and Se(IV) 3.56&nbsp;±&nbsp;0.55&nbsp;ng&nbsp;g-1). In addition, the ratios between selenoproteins and selenometabolites have been calculated for the first to study their potential use as LC biomarkers. The rates eGPx/SEPP1, SEPP1/SeAlb, eGPx/Se(IV) and SEPP1/Se(IV) were significantly different between LC and HC groups. Percutaneous treatments (mainly radiofrequency ablation and ethanol injection) are modes of treatment for hepatocellular carcinoma. Seeding of malignant cells along the percutaneous tract is a very rare complication. We present a case of chest wall metastasis caused by radiofrequency ablation appearing years after liver transplantation. BACKGROUND Sarcopenia (SP) and preoperative muscle mass are independent predictive factors for short- and long-term outcome of liver transplantation. OBJECTIVE To assess the influence of muscle mass index (MMI) and preoperative SP on the prognosis of patients who underwent liver transplantation in our hospital. METHODS Ninety-seven patients who underwent liver transplantation in the Regional University Hospital of Málaga from September 2013 to March 2016 were analyzed. SP was determined based on the MMI, as assessed by psoas muscle area at the L4 level measured by computed tomography (CT), with adjustment for patient sex. https://www.selleckchem.com/products/cirtuvivint.html Two cohorts were differentiated 54 patients without SP and 42 patients with SP. Postoperative complications, graft survival, and patient survival were assessed. A 3-year follow-up was carried out. RESULTS Recipient characteristics were similar in both cohorts, except for MMI ± SD (group without SP 94.03 ± 15.43 cm2/m2 vs group with SP 56.99 ± 13.59 cm2/m2; P&nbsp;=&nbsp;.001). The incidence of postoperative complications (Clavien&nbsp;? 3) in patients with and without SP was 39.5% and 24.1%, respectively (P&nbsp;= .08). SP was not associated with poorer long-term graft or patient survival. CONCLUSIONS SP, determined by preoperative measurement of MMI, was identified as a predictive factor associated with a higher incidence of postoperative complications. Since MMI can be easily determined by CT, it should be assessed in all candidates for liver transplantation. Published by Elsevier Inc.INTRODUCTION New transplant-related therapies such as xenotransplantation of solid cells, tissues, and organs are still poorly understood, especially in countries without preclinical xenotransplantation programs. OBJECTIVE Analyze the attitude toward xenotransplantation among the population of Santiago de Cuba. METHOD Type of study. Cross-sectional observational study. SAMPLE Random sampling of 455 individuals residing in Santiago de Cuba. Assessment instrument. Validated questionnaire of attitude toward the xenotransplant, "PCID-XenoTx-Ríos." Field work. Random selection based on stratification. Anonymous and self-administered completion. RESULTS A total of 49.9% would accept a solid organ xenotransplant if they needed it and the results were similar to those currently obtained with human organs, 27.5% have doubts, and the remaining 22.6% would not accept it. They are presented as statistically significant variables (P&nbsp; less then .05) level of studies; comment with the family about the donation; opinion of a partner; religion; opinion of religion; performing prosocial activities; attitude toward donation; previous relationship with the organ donation and transplant; favorable attitude toward live kidney donation. In the multivariate analysis, the following persist 1. level of university studies odds ratio (OR)&nbsp;= 5.076; 2. not having a partner OR&nbsp;= 5.154; 3. belonging to another religion OR&nbsp;= 2.061; 4. favorable attitude toward organ donation OR&nbsp;= 5; and 5. favorable attitude toward living donation OR&nbsp;= 3.759. CONCLUSIONS The population of Santiago de Cuba is less sensitized toward xenotransplantation, possibly due to ignorance of the issue due to lack of preclinical trials in their countries. INTRODUCTION In liver transplantation, living donation shows better and better results. However, rates of living liver donation (LLD) are low. OBJECTIVE To analyze the attitude towards LLD among patients on the waiting list for liver transplantation. METHOD Study population Patients included in the waiting list for liver transplantation who are not in code 0 and do not present with encephalopathy above grade I. PROTOCOL After being included in the waiting list, they are informed of the study and they are cited in the Psychology Consultation. INSTRUMENTS attitude questionnaire towards LLD, carried out in the Psychological Care consultation. RESULTS Of the 147 patients included in the waiting list, 112 fulfilled the inclusion criteria in the study. After being cited in the consultation, 100% of respondents are in favor of living liver donation. However, when considering the related LLD, 80% of the respondents indicated that they would not accept a living donation from a family member and therefore did not want to complete the questionnaire.